Next Generation Sequencing

FYR Diagnostics is pleased to be able to offer next-generation sequencing (NGS) as a service!

We are dedicated to helping you identify the right NGS strategy and will provide custom services including library preparation and sequencing for a variety of applications at a competitive price.

What is NGS?

Example NGS Workflow

NGS Applications

SARS-CoV-2 Variant Sequencing

FYR is currently offering sequencing of SARS-CoV-2 variants from clinical respiratory samples

FYR has the ability to sequence whole SARS-CoV-2 genomes in order to identify variants of concern or interest. This newly acquired capability also places FYR in a position to handle other sequencing needs for the state of Montana in a diversity of fields such as agriculture, food and beverage, etc. 

Interested or want to know more? Use the contact form below to get started!

Mutations, Strains, Variants, and Lineages

mutation is a change in the genetic material (the genome) of a cell of a living organism or of a virus that is more or less permanent and that can be transmitted to the cell’s descendants or the viral progeny.

While mutations are a major contributor to evolution they are not always meaningful and in the case of pathogens not always a scary thing. Mutations happen all the time, both in microbes and larger organisms such as humans. Additionally, most mutations don’t actually result in any noticeable change. This is because mutation is a random process. Randomly changing nucleotides in the genome of any organism or virus will much more often be deleterious than beneficial. However, because of the constancy of mutation in all organisms some mutations will arise which may bolster the fitness of an organism or virus.

Strain is a bit of a disputed term without clear boundaries. However, it is generally agreed upon that a viral strain is a virus with a distinct genetic makeup and biological properties that differ from other strains or forms of a similar virus.

variant is a genetic lineage or background of a viral strain with a number of mutations consistently seen together which can be tracked and/or alter the behavior of the virus.

A lineage is a series of biological entities or genes that are connected through a line of descent from a common ancestor.

Estimated Proportions of SARS-CoV-2 Lineages

The data below is courtesy of the CDC and shows the estimated biweekly proportions of the most common SARS-CoV-2 lineages circulating in the United States, based on greater than 175,000 sequences collected through CDC’s national genomic surveillance since Dec 20, 2020 and grouped in 2-week intervals.

Data are subject to change over time and will be updated as more data become available. Variant proportions are adjusted using statistical weighting to correct for the non-random sampling of sequencing data over time and across states and to provide more representative national estimates.

More Sequencing Updates

NCBI Virus

Link

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